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Center For Infectious Diseases and Immunology

Otitis Media Epidemiology, Microbiology, Molecular Biology

  • Kaur R, Adlowitz DG, Casey JR, Zeng M, Pichichero ME. Simultaneous Assay for Four Bacterial Species Including Alloiococcus otitidis Using Multiplex-PCR in Children with Culture Negative Acute Otitis Media. Pediatr Infect Dis J 2010; 29:741-745. DOI: 10.1097/INF.0b013e3181d9e639
  • Casey JR, Adlowitz DG, Pichichero ME. New Patterns in the otopathogens causing acute otitis media six to eight years after introduction of pneumococcal conjugate vaccine. Pediatr Infect Dis J 2010 Apr; 29(4):304-9. DOI: 10.1097/INF.0b013e3181c1bc48
  • Xu Q, Kaur R, Casey JR, Adlowitz DG, Pichichero ME  Zeng M. Identification of Streptococcus pneumoniae and Haemophilus influenzae in culture-negative middle ear fluids from children with acute otitis media by combination of multiplex PCR and multi-locus sequencing typing. Int J Pediatr Otorhinolaryngol 2011; 75:239-244. DOI: 10.1016/j.ijporl.2010.11.008
  • Xu Q, Kaur R, Casey JR, Sabharwal V, Pelton S  Pichichero ME. Nontypeable Streptococcus pneumoniae as an Otopathogen. Diag Micro Infect Dis 2011; 69:200-204. DOI: 10.1016/j.diagmicrobio.2010.09.019
  • Casey JR, Block S, Puthoor P, Hedrick J, Almudevar A, Pichichero ME. A Simple Scoring System to Improve Clinical Assessment of Acute Otitis Media. Clin Pediatr 2011; 50:632-629. DOI: 10.1177/0009922811398391
  • Thomas JC, Figueira M, Fennie KP, Laufer AS, Kong Y, Pichichero ME, Pelton SI  Pettigrew MM. Streptococcus pneumoniae clonal complex 199: genetic diversity and tissue-specific virulence. PloS One. 2011; 6:e18649. DOI: 10.1371/journal.pone.0018649
  • Kaur R, Chang A, Xu Q, Casey JR, Pichichero ME. Phylogenetic relatedness and diversity of non-typeable Haemophilus influenzae in the nasopharynx and middle ear fluid of children with acute otitis media. J Med Microbiol. 2011; 60(Pt 12):1841-1848. DOI:10.1099/jmm.0.034041-0
  • Shaikh N, Hoberman A, Kaleida PH, Rockette HE, Kurs-Lasky M, Hoover H, Pichichero ME, Roddey OF, Harrison C, Hadley JA  Schwartz RH. Otoscopic signs of otitis media. Pediatr Infect Dis J. 2011; 30: 822-6. DOI: 10.1097/INF.0b013e31822e6637
  • Xu Q, Casey JR, Chang A, Pichichero ME. When co-colonizing the nasopharynx haemophilus influenzae predominates over Streptococcus pneumoniae except serotype 19A strains to cause acute otitis media. Pediatr Infect Dis J 2012; 31(6):638-640. DOI:10.1097/INF.0b013e31824ba6f7
  • Casey JR, Block SL, Hedrick J, Almudevar A, Pichichero ME. Comparison of amoxicillin/clavulanic acid high dose with cefdinir in the treatment of acute otitis media. Drugs 2012; 72(15):1991-1997. DOI: 10.2165/11590320-000000000-00000
  • Xu Q, Almudevar A, Casey JR, Pichichero ME. Nasopharyngeal bacterial interactions in children. Emerg Infect Dis 2012; 18(11):1738-1745.  DOI: 10.3201/eid1811.111904
  • Friedel V, Chang A, Wills J, Vargas R, Xu Q, Pichichero ME. Impact of Respiratory Viral Infections on α-Hemolytic Streptococci and Otopathogens in the Nasopharynx of Young Children. Pediatr Infect Dis J 2013; 32(1):27-31. DOI: 10.1097/INF.0b013e31826f6144
  • Pichichero ME, Casey JR, Almudevar A. Reducing the Frequency of Acute Otitis Media by Individualized Care. Pediatr Infect Dis J2013;32(5):473-478. DOI: 10.1097/INF.0b013e3182862b57
  • Pichichero ME. Otitis Media. Pediatr Clin No Am 2013; 60:391-407. DOI: 10.1016/j.pcl.2012.12.007
  • Casey JR, Kaur R, Friedel V, Pichichero ME. Acute Otitis Media Otopathogens during 2008-2010 in Rochester, NY. Pediatr Infect Dis J 2013; 32(8):805-809.  DOI: 10.1097/INF.0b013e31828d9acc
  • Kaur R, Casey JR, Pichichero ME. Relationship with Original Pathogen in Recurrence of Acute Otitis Media after Completion of Amoxicillin/Clavulanate: Bacterial Relapse or New Pathogen. Pediatr Infect Dis J 2013; 32(11):1159-1162. DOI: 10.1097/INF.0b013e31829e3779
  • Friedel V, Zilora S, Bogaard D, Casey JR, Pichichero ME. Five-year prospective study of paediatric acute otitis media in Rochester, NY: modeling analysis of the risk of pneumococcal colonization in the nasopharynx and infection. Epidemiol Infect 2014;142(10):2186-2194.  DOI: 10.1017/S0950268813003178
  • Kaur R, Czup K, Casey JR, Pichichero ME. Correlation of nasopharyngeal cultures prior to and at onset of acute otitis media with middle ear fluid cultures. BMC Infect Dis 2014; 14(1):640.  DOI: 10.1186/s12879-014-0640-y
  • Pichichero ME and Casey JR. Acute Otitis Media: Update 2015. Contemporary Pediatrics 2015; March: 15-18. Pichichero ME. Ten Year Study of Acute Otitis Media. Pediatr Infect Dis J 2016; 35(9):1027-1032. DOI: 10.1097/INF.0000000000001216
  • Kaur R, Morris M and Pichichero ME. Epidemiology of Acute Otitis Media in the Post Pneumococcal Conjugate Vaccine Era. Pediatrics 2017 Sept; 140(3). DOI: 10.1542/peds.2017-4067
  • Xu Q, Wischmeyer J, Gonzalez E and Pichichero ME. Nasopharyngeal polymicrobial colonization during health, viral upper respiratory infection and upper respiratory bacterial infection. J Infect 2017; 75(1):26-34. DOI: 10.1016/j.jinf.2017.04.003
  • Kaur R, Wischmeyer J, Morris M and Pichichero ME. Comparison of direct-plating and broth-enrichment culture methods for detection of potential bacterial pathogens in respiratory secretions. J Med Microbiol 2017; 66(11):1539-1544. DOI: 10.1099/jmm.0.000587

Otitis Media Immunology

  • Liu K, Casey JR, Pichichero ME. Serum Intercellular Adhesion Molecule 1 Variations in Young Children with Acute Otitis Media. Clinical and Vaccine Immunology 2010; 17(12):1909-1916.  DOI: 10.1128/CVI.00194-10
  • Sharma SK, Casey JR, Pichichero ME. Reduced Memory CD4+T-Cell Generation in the Circulation of Young Children May Contribute to the Otitis-Prone Condition. J Inf Dis 2011: 204: 645-653. DOI: 10.1093/infdis/jir340
  • Kaur R, Casey JR, Pichichero ME. Serum antibody response to five Streptococcus pneumoniae proteins during acute otitis media in otitis-prone and non-otitis-prone children.  Pediatr Infect Dis J. 2011; 30:645-650. DOI: 10.1097/INF.0b013e31821c2d8b
  • Pichichero ME, Kaur R, Casey JR, Xu Q, Almudevar A, Ochs M. Antibody response to Streptococcus pneumoniae proteins PhtD, LytB, PcpA, PhtE and Ply after nasopharyngeal colonization and acute otitis media in children. Hum Vaccin Immunother 2012; 8(6) 799-805.  DOI: 10.4161/hv.19820
  • Sharma SK, Casey JR, Pichichero ME. Reduced serum IgG responses to pneumococcal antigens in otitis-prone children may be due to poor memory B-cell generation. J Infect Dis 2012; 205(8):1225-9.  DOI: 10.1093/infdis/jis179
  • Liu K, Pichichero ME. Clinical significance of serum S100A12 in acute otitis media in young children. Pediatr Infect Dis J 2012,31:e56-8. DOI: 10.1097/INF.0b013e31824672cb
  • Liu K, Chen L, Kaur R, Pichichero ME. Transcriptome signature in young children with acute otitis media due to Streptococcus pneumoniae. Microbes Infect. 2012 (7-8):600-609. DOI: 10.1016/j.micinf.2012.01.006
  • Kaur R, Kim T, Casey JR, Pichichero ME. Antibody in Middle Ear Fluid of Children Originates Predominantly from Serum and Nasopharyngeal Secretions. Clin Vaccine Immunol 2012; 19(10):1593-1596. DOI: 10.1128/CVI.05443-11
  • Sharma SK, Pichichero ME. Functional deficits of pertussis-specific CD4+ T cells in infants compared to adults following DTaP vaccination. Clin Exp Immunol 2012; 169(3)281-291. DOI: 10.1111/j.1365-2249.2012.04613.x
  • Sharma SK, Pichichero ME. Deficiencies in the CD4+ T-Helper Cell Arm of the Immune System of Neonates and Young Children. Pediatric Allergy, Immunology and Pulmonology 2013; 26(1):4-10.
  • Sharma SK, Roumanes D, Almudevar A, Mosmann T, Pichichero ME. Divergence in CD4+ T-cell Responses Among Young Children and Adults in Response to Streptococcus pneumoniae and Haemophilus influenzae Vaccine Candidate Protein Antigens. Vaccine 2013; 31(30):3090-3097. DOI: 10.1016/j.vaccine.2013.03.060
  • Verhoeven D, Nesselbush M, Pichichero ME. Lower Nasopharyngeal Epithelia Cell Repair and Diminished Innate Inflammation Responses Contribute to the Onset of Acute Otitis Media. Med Microbiol Immuno 2013; 202(4):295-302. DOI: 10.1007/s00430-013-0293-2
  • Pichichero ME, Casey JR, Almudevar A. Non-Protective Responses to Pediatric Vaccines Occur in Children Who are Otitis Prone. Pediatr Infect Dis J 2013;32 (11):1163-1168. DOI: 10.1097/INF.0b013e31829e887e
  • Sharma SK and Pichichero ME. Cellular Immune Response in Young Children Accounts for Recurrent Acute Otitis Media. Curr Allergy Asthma Rep 2013; 13(5):495-500.  DOI: 10.1007/s11882-013-0370-z
  • Xu Q and Pichichero ME. Co-colonization by Haemophilus influenza with Streptococcus pneumonia enhances Pneumococcal specific antibody response in young children. Vaccine 2014; 32(6):706-711. DOI: 10.1016/j.vaccine.2013.11.096
  • Verhoeven D, Xu Q, Pichichero ME. Differential Impact of Respiratory Syncytial Virus and Parainfleunza Virus on the Frequency of Acute Otitis Media is Explained by Lower Adaptive and Innate Immune Responses in Otitis-Prone Children. Clin Infect Dis 2014;59(3):376-383.  DOI: 10.1093/cid/ciu303
  • Verhoeven D, Pichichero ME. Divergent mucosal and systemic responses in children in response to acute otitis media. Clin Exp Immunol 2014; 178(1):94-101.  DOI: 10.1111/cei.12389
  • Kaur R, Casey JR, Pichichero ME. Cytokine, Chemokine, and Toll-Like Receptor Expression in Middle Ear Fluids of Children with Acute Otitis Media. Laryngoscope 2015 Jan;125(1):E39-E44. DOI: 10.1002/lary.24920
  • Ren D, Almudevar A, Pichichero ME. Synchrony in serum antibody response to conserved proteins of Streptococus pneumonia in young children. Human Vacc and Immunol 2015; 11(2):489-497.  DOI: 10.4161/21645515.2014.990861
  • Xu Q, Casey JR, Pichichero ME. Higher Levels of Mucosal Antibody to Pneumococcal Vaccine Candidate Proteins Correlate with Reduced Acute Otitis Media Caused by Streptococcus pneumonia in Young Children.  Mucosal Immunol 2015;182(3):314-322.  DOI: 10.1038/mi.2015.1
  • Basha S and Pichichero ME. Poor Memory B cell generation contributes to Nonprotective Responses to DTaP vaccine antigens in Otitis-Prone Children. Clin Exp Immunol 2015; 182(30):314-322.  DOI: 10.1111/cei.12660
  • Morris M, Almudevar A, Casey JR and Pichichero ME. Familial and Microbiological Contribution to the Otitis-Prone Condition. Int J Pediatr Otorhinalarngal 2015;79(12):2174-2177.  DOI: 10.1016/j.ijporl.2015.09.043
  • Surendran N, Nicolosi T, Kaur R and Pichichero ME. Peripheral blood antigen presenting cell responses in otitis-prone and non-otitis prone infants. Innate Immun 2016;22(1):63-71.  DOI: 10.1177/1753425915616267
  • Xu Q, Casey JR, Newman E and Pichichero ME. Otitis-Prone Children have Immunologic Deficiencies in Naturally Acquired Nasopharyngeal Mucosal Response after Streptococcus pneumoniae colonization. Pediatr Infect Dis J 2016; 35(1):54-60. DOI: 10.1097/INF.0000000000000949
  • Morris M, Kozara K, Salamone F, Benoit M and Pichichero ME. Adenoidal follicular T helper cells provide stronger B cell help than those from tonsils. Laryngoscope 2016 Feb;126(2):E80-85. DOI: 10.1002/lary.25536
  • Pichichero ME. Ten Year Study of the Stringently Defined Otitis Prone Child. Pediatr Infect Dis J 2016; 35(9):1033-1039. DOI: 10.1097/INF.0000000000001217
  • Kaur R, Casey JR and Pichichero ME. Differences in innate immune response gene regulation in the middle ear of children who are otitis prone and in those not otitis prone. Am J Rhino Allergy 2016; 30(6):e218-e223. DOI: 10.2500/ajra.2016.30.4393
  • Basha S, Kaur R, Mosmann T and Pichichero ME. Reduced T helper 17 responses to Streptococcus pneumonia in infection-prone children can be rescued by addition of innate cytokines. J Infect Dis 2017; 215(8):1321-1330. DOI: 10.1093/infdis/jix090
  • Kyd JM, Hotomi M, Korio M, Kurali A, Pichichero M, Ryan A, Swords WE and Thorton R. Panel 5: Immunology. Otolaryngol Head Neck Surg 2017; 156(4_Suppl): S63-S75. DOI: 10.1177/0194599816663886
  • Morris M, Khan MN, Pichichero ME. A PCR-based method for quantifying neutrophils in human nasal secretions. J of Immunological Methods 2017;447: 65-70.  DOI: 10.1016/j.jim.2017.04.010
  • Basha S and Pichichero ME. Decreased TNF family receptor expression on B-cells is associated with reduced humoral responses to Streptococcus pneumonia infections in young children. Cell Immunol 2017; 320(1):11-19. DOI: 10.1016/j.cellimm.2017.07.004
  • Morris M and Pichichero ME. Streptococcus pneumonia burden and nasopharyngeal inflammation during acute otitis media. Innate Immunity 2017; 23(8):667-677. DOI: 10.1177/1753425917737825
  • Ren D, Xu Q, Almudevar A, Pichichero ME. Impaired proinflammatory response in stringently defined otitis prone children during viral upper respiratory infections. Clin Infect Dis 2019 May 68(9); 1566-1574  DOI: 10.1093/cid/ciy750

Haemophilus Influenza Vaccine

  • Pichichero ME, Kaur R, Casey JR et al. Antibody response to Haemophilus influenzae outer membrane protein D, P6, and OMP 26 after nasopharyngeal colonization and acute otitis media in children. Vaccine 2010; 28:7184-7192. DOI: 10.1016/j.vaccine.2010.08.063
  • Kaur R, Casey JR, Pichichero ME. Serum Antibody Response to Three Nontypeable Haemophilus influenzae Outer Membrane Proteins During Nasopharyngeal Colonization and Acute Otitis Media in Otitis Prone and Non Otitis Prone Children. Vaccine 2011; 29:1023-1028. DOI: 10.1016/j.vaccine.2010.11.055
  • Michel LV, Kalmeta B, McCreary M, Snyder J, Craig P, Pichichero ME. Vaccine Candidate P6 on Nontypeable Haemophilus influenzae is Not a Trans membrane Protein Based on Protein Structural Analysis. Vaccine Feb 11; 29:1624-1627. DOI: 10.1016/j.vaccine.2010.12.082
  • Chang A, Kaur R, Michel LV, Casey JR, Pichichero ME. Haemophilus influenzae Vaccine Candidate Outer Membrane Protein P6 is Not Conserved in All Strains. Human Vaccines 2011; 7(1) 102-105. DOI: 10.4161/hv.7.1.13351
  • Khan MN, Kaur R, Pichichero ME. Bactericidal antibody response against P6, protein D and OMP26 of nontypeable Haemophilus influenzae after acute otitis media in otitis-prone children. FEMS Immunol Med Microbiol 2012; 65(3):439-447. DOI: 10.1111/j.1574-695X.2012.00967.x
  • Michel L, Snyder J, Schmidt R, Milillo J, Grimaldi K, Kalmeta B, Khan MN, Sharma S, Wright LK  Pichichero ME. Dual Orientation of the Outer Membrane Lipoprotein P6 in Nontypable Haemophilus influenza. J of Bacteriology 2013; 195(14): 3252-3259. DOI: 10.1128/JB.00185-13
  • Khan MN, Ren R, Kaur R, Basha S, Zagursky R and Pichichero ME. Developing a vaccine to prevent otitis media caused by Nontypeable Haemophilus influenza. Expert Review of Vaccines 2016; 15(7):863-878. DOI: 10.1586/14760584.2016.1156539
  • Michel LV, Ravinder K, Zavorin M, Pryharski K, Khan M, LaClair C, O’Neil M, Xu Q, Pichichero ME. Intranasal coinfection model allows for assessment of protein vaccines against nontypeable Haemophilus influenzae in mice. J Med Microbiol. 2018; 67(10):1527-1532. DOI:10.1099/jmm.0.000827

Pneumococcal Vaccine

  • Khan MN, Pichichero ME. Vaccine candidates PhtD and PhtE of Streptococcus pneumoniae are adhesions that elicit functional antibodies in humans. Vaccine 2012;30(18):2900-7. DOI: 10.1016/j.vaccine.2012.02.023
  • Khan MN, Sharma SK, Filkins LM, Pichichero ME. PcpA of Streptococcus pneumoniae mediates adherence to nasopharyngeal and lung epithelial cells and elicits functional antibodies in humans. Microbes Infect 2012; 14(12):11021110.  DOI: 10.1016/j.micinf.2012.06.007
  • Khan MN, Pichichero ME. CD4T cell memory and antibody responses against pneumococcal histidine triad proteins PhtD, and PhtE following vaccination and colonization and their role in protection against pneumococcal nasopharyngeal colonization in mice. Infect and Immun 2013; 81(10):3781-3792. DOI: 10.1128/IAI.00313-13
  • Verhoeven D, Perry S, Pichichero ME. Contributions to Protection from Streptococcus pneumonia Infection Using Monovalent Recombinant Protein Vaccine Candidates PcpA, PhtD and PlyD1 in an Infant Murine Model during Challenge. Clin Vacccine Immunol 2014; 21(8):1037-1045.  DOI: 10.1128/CVI.00052-14
  • Verhoeven D, Xu Q, Pichichero ME. Vaccination with a Streptococcus pneumonia trivalent recombinant PcpA, PhtD, PlyD1 protein vaccine candidate protects against lethal pneumonia in an infant murine model. Vaccine 2014; 32(26):3205-3210. DOI: 10.1016/j.vaccine.2014.04.004
  • Kaur R, Surendran N, Ochs M, Pichichero ME. Human antibodies to PhtD, PcpA, and Ply reduce adherence to human lung epithelial cells and murine nasopharyngeal colonization by Streptococcus pneumonia. Infect Immun 2014; 82(12):5069-5075. DOI: 10.1128/IAI.02124-14
  • Khan N, Pichichero ME. The Host Immune Dynamics of Pneumococcal Colonization: Implications for Novel Vaccine Development. Human Vacc and Immunol 2014; 10(12)3688-3699. DOI: 10.4161/21645515.2014.979631
  • Xu Q, Surendran N, Verhoeven D, Klapa J, Ochs M, Pichichero ME. Trivalent pneumococcal protein recombinant vaccine protects against lethal Streptococcus pneumonia and correlates with phagocytosis by neutrophils during early pathogenesis. Vaccine 2015;33(8):993-1000. DOI: 10.1016/j.vaccine.2015.01.014
  • Pichichero ME, Khan N and Xu Q. Next generation protein based Streptococcus pneumoniae vaccines. Hum Vaccin Immunother 2016;12(1):194-205. DOI: 10.1080/21645515.2015.1052198
  • Kaur R, Casey JR and Pichichero ME. Emerging Streptococcus pneumonia strains colonizing the Nasopharynx in Children after 13 Valent (PCV13) Pneumococcal Conjugate Vaccination in Comparison to the 7-Valent (PCV7) Era. Pediatr Infect Dis J 2016;35(8):901-906. DOI: 10.1097/INF.0000000000001206
  • Khan MN, Xu Q and Pichichero ME. Protection Streptococcus pneumonia Invasive Pathogenesis by a Protein-Based Vaccine is Achieved by Suppression of Nasopharyngeal Bacterial Density during Influenza A Virus Coinfection.  Infect Immun. 2017; 85(2). DOI: 10.1128/IAI.00530-16
  • Xu Q, Pryharski K and Pichichero ME. Trivalent Pneumococcal Protection Vaccine Protects against Experimental Acute Otitis Media Caused by Streptococcus pneumonia in an Infant Murine Model. Vaccine 2017; 35(2): 337-344. DOI: 10.1016/j.vaccine.2016.11.046
  • Xu Q, Casey JR, Almudevar A and Pichichero ME. Correlation of Higher Antibody Levels to Pneumococcal Proteins with Protection from Pneumococcal Acute Otitis Media but not Protection from Nasopharyngeal Colonization in Young Children. Clin Microbiol Infect. 2017 ; 23 (7):487 DOI: 10.1016/j.cmi.2017.01.011
  • Pichichero ME. Pneumococcal Whole-Cell and Protein Based Vaccines: Changing the Paradigm. Expert Review of Vaccines. 2017;16(12):1181-1190.  DOI: 10.1080/14760584.2017.1393335
  • Abraham C, Pichichero ME, Eisenberg J and Singh S. Third-Trimester Maternal Vaccination Against Pertussis and PertussisAntibody Concentrations. Obstet Gynecol 2018; 131:364-369.  DOI: 10.1097/AOG.0000000000002438
  • Pichichero M, Kaur R, Scott DA, Gruber WC, Trammel J, Almudevar A and Center K. Effectiveness of 13-valent pneumococcal conjugate vaccination for protection against acute otitis media caused by Streptococcus pneumoniae in healthy young children: a prospective observational study. Lancet Child Adolesc Health. 2018; 2(8);561-568. DOI: 10.1016/S2352-4642(18)30168-8
  • Khan MN, Pryharski K, Pichichero, ME. Immunogenicity and protective efficacy of monovalent PCVs containing 22F and 33F polysaccharides in mouse models of colonization and co-infection. Vaccine 2018; 36 (38): 5701-5708. DOI: 10.1016/j.vaccine.2018.08.017

Moraxella Catarrhalis Vaccine

  • Ren D and Pichichero ME. Vaccine targets against Moraxella catarrhalis. Expert Opin Ther Targets 2015; 23(1):1-15. DOI: 10.1517/14728222.2015.1081686
  • Ren D, Almudevar A, Murphy TF, Lafontaine ER, Campagnari AA, Luke –Marshall N, Casey JR and Pichichero ME. Serum antibody response to Moraxella catarrhalis proteins OMP CD, OppA, Msp22, Hag and PilA2 after nasopharyngeal colonization and acute otitis media in children. Vaccine 2015; 33(43):5809-5814.  DOI: 10.1016/j.vaccine.2015.09.023
  • Ren D, Almudevar A, Murphy TF, Lafontaine ER, Campagnari AA, Luke-Marshall, and Pichichero ME. Serum Antibody Response to Moraxella Catarrhalis Proteins in Stringently Defined Otitis Prone Children.  Vaccine 2019; 37(32):4637-4635. DOI: 10.1016/j.vaccine.2017.07.027
  • Ren D, Murphy TF, Lafontaine ER and Pichichero ME. Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins. Front Immunol 2017; 8:953. DOI: 10.3389/fimmu.2017.00953

Vaccine Immunology

  • Baxter R, Jeanfreau R, Block SL, Blatter M, Pichichero ME, et al. A Phase III Evaluation of Immunogenicity and Safety of Two Trivalent Inactivated Seasonal Influenza Vaccines in US Children. Pediatr Infect Dis J 2010; 29:924-930. DOI: 10.1097/INF.0b013e3181e075be
  • Gall SA, Myers J, Pichichero ME. Maternal immunization with tetanus-diphtheria-pertussis vaccine: effect on maternal and neonatal serum antibody levels. Am J Obstet Gynecol. 2011;204(4):334.e1-5. DOI: 10.1016/j.ajog.2010.11.024
  • Wheeler CM, Harvey BM, Pichichero ME, Simon MW, Combs SP, Blatter MM, Marshall GS, Catteau G, Dobbelaere K, Descamps D, Dubin G, Schuind A. Immunogenicity and Safety of Human Papillomavirus-16/18 AS04-adjuvanted Vaccine Coadministered with Tetanus Toxoid Reduced Diphtheria Toxoid, and Acellular Pertussis Vaccine and/or Meningococcal Conjugate Vaccine to Healthy Girls 11 to 18 Years of Age: Results From a Randomized Open Trial. Pediatr Infect Dis J. 2011; 30: e225-34. DOI: 10.1097/INF.0b013e31822d28df
  • Pichichero ME. Protein carriers of conjugate vaccines: Characteristics, development and clinical trials. Human Vaccine Immunother 2013; 9(12) 2505-2523.  DOI: 10.4161/hv.26109
  • Pichichero ME. Challenges in vaccination of neonates, infants and young children. Vaccine 2014; 32(31):3886-3894. DOI: 10.1016/j.vaccine.2014.05.008
  • Basha S, Surendran N, Pichichero ME. Immune response in neonates. Expert Review of Clinical Immunology 2014; 10(9):1171-1184.  DOI: 10.1586/1744666X.2014.942288
  • Pichichero ME, Casey JR, Almudevar A, Basha S, Surendran N, Kaur R, Morris M, Livingstone A and Mosmann T. Functional Immune Cell Differences Associated with Low Vaccine Responses in Infants. J Infect Dis 2016; 213(12):2014-2019. DOI: 10.1093/infdis/jiw053
  • Robertson CA, Greenberg DP, Hedrick J, Pichichero ME, Decker MD and Saunders M. Safety and Immunogenicity of a Booster Dose of Meningococcal (Groups A, C, W and Y) Polysaccharide Diphtheria Toxoid Conjugate Vaccine. Vaccine 2016; 34(44):5273-5278.  DOI: 10.1016/j.vaccine.2016.09.003
  • Surendran N, Nicolosi T and Pichichero M. Infants with low vaccine antibody responses have altered innate cytokine response. Vaccine 2016; 34:5700-5703.  DOI: 10.1016/j.vaccine.2016.09.050
  • Surendran N, Nicolosi T, Kaur R, Morris M and Pichichero ME. Prospective study of innate cellular immune response in low vaccine responder children. Innate Immun 2017; 23(1):89-96. DOI: 10.1177/1753425916678471
  • Surendran N, Simmons A and Pichichero ME. TLR agonist combinations that stimulate Th type I polarizing responses from human neonates. Innate Immun 2018; 24(4):240-251. DOI: 10.1177/1753425918771178
  • Surendran N, Pichichero M. Genetically detoxified pertussis toxin induces superior antigen specific CD4 T cell responses compared to chemically detoxified pertussis toxin. Hum Vaccin Immunother 2019; 15(5):1167-1170. DOI: 10.1080/21645515.2019.1565270


  • Liu K, Almudevar A and Pichichero ME. Higher serum levels of interleukin 10 occur at onset of acute otitis media caused by Streptococcus pneumoniae compared to Haemophilus influenzae and Moraxella catarrhalis. Laryngoscope 2013; 123(6): 1500-1505. DOI: 10.1002/lary.23973
  • Liu K, Chen LL, Kaur R, Pichichero ME. Transcriptome signature in young children with acute otitis media due to Nontypeable Haemophilus influenzae. Int Immunol 2013; 25(6):353-361. DOI: 10.1093/intimm/dxs154 
  • Pichichero ME and Almudevar A. Serum Cytokine Biomarkers Accurately Predict Presence of Acute Otitis Media Infection and Recovery caused by Nontypeable Haemophilus influenzae. Int J Pediatr Otorhinolaryngol 2016; 83(1):200-204. DOI: 10.1016/j.ijporl.2016.02.007
  • Pichichero ME and Almudevar A. Inflammation Associated Cytokine Analysis Identifies Presence of Respiratory Bacterial Pathogens in the Nasopharynx.  Pathog Dis. 2016 Aug; 74(6).  DOI: 10.1093/femspd/ftw064
  • Pichichero ME, Morris MC and Almudevar A. Three Innate Cytokine Biomarkers Predict Presence of Acute Otitis Media and Relevant Otopathogens. Biomark Applic 2018; BMAP-118.


  • Vaidya K, Osgood R, Ren D, Pichichero M, Helguera M. “Ultrasound Imagine and Characterization of Biofilms Based on Wavelet Denoised RF Data”. Ultrasound Med Bio 2014; 40(3):583-595.  DOI: 10.1016/j.ultrasmedbio.2013.11.005
  • Osgood R, Salamone F, Diaz A, Casey JR, Pichichero ME. Effect of pH and Oxygen on Biofilm Formation in Acute Otitis Media Associated NTHi Clinical Isolates. Laryngoscope 2015;125(9):2204-2208.  DOI: 10.1002/lary.25102


  • Pichichero ME and Almeduvar A. Modeling Specific Antibody Responses to Natural Immunization to Predict a Correlate of Protection against Infection before Commencing a Clinical Vaccine Trial. Human vaccine and Immunother 2017; 13(10):2316-2321. DOI: 10.1080/21645515.2017.1329064
  • Almudevar A and Pichichero ME. Haemophilus influenza-Protein D Specific Antibody Correlate with Protection against Acute Otitis Media in Young Children. Vaccine 2018; 36(9):1133-1135. DOI: 10.1016/j.vaccine.2018.01.021 
  • Sedghamiz H, Morris M, Whitley D, Craddock TJA, Pichichero M, Broderick G.  Computation of Robust Minimal Intervention Sets in Multi-valued Biological Regulatory Networks.  Front Physiol. 2019; 10(241); 1-14. DOI: 10.3389/fphys.2019.00241
  • Almudevar A, Kaur R, Pichichero M. Statistical projection of post-vaccination antibody kinetics between dosing schedules. Vaccine 2019; 37(32) 4561-4567. DOI: 10.1016/j.vaccine.2019.06.017


  • Xu Q, Gill S, Xu L, Gonzalez E, Pichichero, M. Comparative Analysis of Microbiome in Nasopharynx and Middle Ear in Young Children With Acute Otitis Media.  Front Genet 2019: 10(1176); 1-7. DOI: 10.3389/fgene.2019.01176